This form is for MHC Class II requests only

Click here for MHC Class I request form

The request form is used for review of the request.

** Note: One request form per tetramer

Important! Your request will not be considered unless all the fields on this form are filled.

(Click here to find out when the Tetramer Resource Committee meets again to review requests.)

Be sure to register with our reagent program if you are a new client.

(Click here to download the Registration Form)

Contact and Shipping Information

Investigator's Name:
Title:
Institution:
Department:
Street Address:
City, State, ZIP
Telephone Number:
Fax Number:
E-mail: (Please note: our system can only accept one email address here on this form.)
Shipping Company / Account No.
All reagents will be shipped to the above address which cannot be a post office box.

Currently Available Pre-Made MHC Class II Monomers and Tetramers

Designation	Allele	Source	Peptide	Fluorophore
I-A(b) gp 66-77	I-A(b)	lymphocytic choriomeningitis virus glycoprotein 66-77	DIYKGVYQFKSV	PE or APC
I-A(b) MOG 38	I-A(b)	mouse myelin oligodendrocyte glycoprotein 38-49	GWYRSPFSRVVH	PE or APC
I-A(b) Ova 323	I-A(b)	chicken ovalbumin 323-339 Please note: This Ova reagent is problematic. Very high concentrations of tetramer are required,and it still may not stain 100% of the appropriate cells. We recommend using another reagent or model system if possible.	ISQAVHAAHAEINEAGR	PE or APC
I-A(b) ESAT-6	I-A(b)	M. tuberculosis 6kDa early secretory antigenic target 1-20	MTEQQWNFAGIEAAASAIQG	PE or APC
I-A(b) Ag85B	I-A(b)	M. tuberculosis antigen 85-B precursor 280-294	FQDAYNAAGGHNAVF	PE or APC
I-A(b) LTAg	I-A(b)	murine polyomavirus large T antigen 676-688	NEYLLPQTVWARF	PE or APC
I-A(b) FMLV	I-A(b)	Friend murine leukemia virus envelope protein 123-141	EPLTSLTPRCNTAWNRLKL	PE or APC
I-A(b) Ea	I-A(b)	mouse MHC class II antigen E alpha 50-66	ASFEAQGALANIAVDKA	PE or APC
I-A(b) MHV	I-A(b)	murine hepatitis virus membrane protein M 133-147	TVYVRPIIEDYHTLT	PE or APC
I-A(b) RSV M	I-A(b)	respiratory syncytial virus matrix protein M 209-223	NKGAFKYIKPQSQFI	PE or APC
I-A(b) Flag	I-A(b)	uncultured bacterium flagellin 456-475	DMATEMVKYSNANILSQAGQ	PE or APC
I-A(b) tul4	I-A(b)	francisella tularensis 17kD lipoprotein tul4 86-99	RLQWQAPEGSKCHD	PE or APC
I-A(b) Cta1	I-A(b)	chlamydia trachomatis predicted periplasmic protein Cta1 133-152	KGIDPQELWVWKKGMPNWEK	PE or APC
I-A(b) RSV M2	I-A(b)	human respiratory syncytial virus transcriptase protein matrix protein 2 26-39	NYFEWPPHALLVRQ	PE or APC
I-A(d) HA	I-A(d)	influenza A virus hemagluttinin 141-155	RGHNVNGVTAACSHE	PE or APC
I-A(d) malE	I-A(d)	maltose binding protein malE 69-84	GYAQSGLLAEITPDKA	PE or APC
I-A(d) Ova 323	I-A(d)	chicken ovalbumin 323-339	ISQAVHAAHAEINEAGR	PE or APC
I-A(d) PLL	I-A(d)	mouse H+/K+ ATPase 889-900	PLLCVGLRPQWE	PE or APC
I-A(d) PIT	I-A(d)	mouse H+/K+ ATPase 630-641	PITAKAIAASVG	PE or APC
I-A(g7) BDC 2.5 seq 1	I-A(g7)	BDC 2.5 mimotope sequence 1	AHHPIWARMDA	PE or APC
I-A(g7) BDC 2.5 seq 2	I-A(g7)	BDC 2.5 mimotope sequence 2	RTRPLWVRME	PE or APC
I-A(g7) BDC 15	I-A(g7)	BDC 15 mimotope	AAAAVRPLWVRMEAA	PE or APC
I-A(g7) Ova	I-A(g7)	chicken ovalbumin 141-160	CARELINSWVESQTNGIIRN	PE or APC
I-A(g7) CD23	I-A(g7)	rat CD23 216-231	DLKGRLVSIHSQKEQD	PE or APC
I-A(g7) E25B	I-A(g7)	rat/mouse E25B 112-126	YQTIEENIKIFEEDA	PE or APC
I-A(g7) GAD65	I-A(g7)	human glutamic acid decarboxylase 2 (65kDa) 524-543	SRLSKVAPVIKARMMEYGTT	PE or APC
I-A(g7) PLP	I-A(g7)	mouse proteolipid protein 56-70	DYEYLINVIHAFQYV	PE or APC
DRB1*0301 PLP	DRB1*0301	human myelin proteolipid protein 97-108	RQIFGDYKTTIC	PE or APC
DRB1*0301 hsp	DRB1*0301	Mycobacterium heat shock protein 65 3-13	KTIAYDEEARR	PE or APC
DP1*0401 TTX	DP1*0401	clostridium tetani tetanus toxin 947-967	FNNFTVSFWLRVPKVSASHLE	PE or APC
DP1*0401 Oxy	DP1*0401	human oxytocinase 272-284	KKYFAATQFEPLA	PE or APC
DQB105 gag	DQB105	human immunodeficiency virus 1 gag protein 293-303	FRDYVDRFYKT	PE or APC
DQB105 FcR	DQB105	human Fc fragment of IgE (Rc for CD23) 104-122	SQDLELSWNLNGLQADLSS	PE or APC
Patr DRB1*0701 NS4	Patr DRB1*0701	hepatitis C virus NS4 1790-1799	LMAFTAAVTS	PE or APC
Patr DRB1*1001 NS3	Patr DRB1*1001	hepatitis C virus NS3 1248-1258	GYKVLVLNPSV	PE or APC

One order contains 200 ug of class II protein.

Tetramer Requested

Funding and Project Information

FOR NEW AND REFILL REQUESTS: PLEASE COMPLETE ALL FIELDS BELOW (failure to complete all fields will delay review and approval process).�(NIH funding is not a requisite for requesting tetramers)

Title of Current Research Grant(s) or Contract supported by NIH for which tetramers will be used:
Grant or Contract Number (if any):
Please list the staff that will be using this reagent (include collaborators):
Positive Control to test the requested tetramer(s): Yes No Please describe the positive control that you will use to test the requested tetramer. If you do not have a positive control, please describe the method you will use to determine that the tetramer is working in your laboratory.
Research Abstract: This abstract should include a brief description of the research project, experimental system, how the tetramer reagent will be used to facilitate the research project, and a justification as to why tetramer results are needed instead of or in addition to results obtained from assays such as cytokine ELISPOTS or cytokine capture assays that do not require precise identification of MHC restriction. (limit: 1000 words)

If this is a refill request, please also answer the following questions.

Have you submitted a feedback form on your original order?
If you have not provided feedback to the NIH Tetramer Facility Manager, approval of your order could be delayed.
Please go to our online feedback form and submit a complete report.
Please provide your reason for requesting additional tetramer. (If the tetramer no longer works, please provide a brief description of how the tetramer was stored and explain the experimental conditions in which the tetramer failed to stain, including the positive and negative controls that were used in the study.)

Last updated 08/18/08